Reporter
lacZ a

Part:BBa_E0033:Experience

Designed by: Yong-Su Jin (Fighting Darwins)   Group: Antiquity   (2004-01-27)

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Please enter how you used this part and how it worked out.

Applications of BBa_E0033

User Reviews

UNIQ2a92b7af0dc1a1ee-partinfo-00000000-QINU

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RS

  • RS 11:45, 20 July 2007 (EDT): As currently specified, this coding region lacks an in frame stop codon. There should be an additional T before the AATAA (last 5 nucleotides) in order for the double stop codon to be in frame with the rest of the protein. Sequencing of BBa_E0433 indicates that the sequence in the Registry does reflect the physical part sequence. So the physical part also lacks an in frame stop codon. This may render lacZalpha nonfunctional.

UNIQ2a92b7af0dc1a1ee-partinfo-00000002-QINU